BIOL2499/BIOL2512 Systems Biology – Journal Club Part C
Question 1:
Meyer et al. 2021. Nature volume 590, pages 284–28
Explain why Meyer et al (2021) used a combination of Illumina short read DNA and RNA next generation sequencing (NGS) and Oxford Nanopore long read DNA NGS to assemble and annotate the genome of the Australian lungfish.
Question 2:
Miller et al. 2021 Genet. 108(8):1436-1449.
Discuss how and why Miller et al (2021) used long-read NGS technology to overcome the limitations of short-read NGS technology in human clinical testing.
Question 3:
Quispe-Parra et al, 2021. Sci Rep. 11(1):346.
3 samples of RNA were extracted from groups of pooled organisms (sea cucumbers) at each stage (normal, day 1 and day 3) and RNA-sequencing was performed using Illumina NGS.
Explain why 3 RNA extractions (rather than a single extraction) were used from each stage.
Question 4:
Junker et al. 2019. PLoS Pathog 15(5):e1007692.
Describe how genomics, transcriptomics and proteomics were used to show that the sulphur metabolic process (including methionine) plays an important role in the predatory behaviour of S. schoenii.
