Outline the process of molecular cloning of a gene or segment of DNA
Describe appropriate methods for cloning eukaryotic genes so their protein products can be expressed (transcribed into mRNA, which is then translated to make protein).
Compare and contrast the vectors and procedures used for creating genetically modified bacteria and eukaryotes
Describe genome editing with CRISPR-Cas9 – what is required for targeting and how target genes can be modified
